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Figure 4 | Breast Cancer Research

Figure 4

From: Transducin (β)-like 1 X-linked receptor 1 promotes proliferation and tumorigenicity in human breast cancer via activation of beta-catenin signaling

Figure 4

Transducin (β)-like 1 X-linked receptor 1 (TBLR1) silencing inhibits cell proliferation and G1-S phase transition in breast cancer cells. (A) Western blotting shows the levels of TBLR1 protein in TBLR1-silenced breast cancer cells; glyceraldehyde-3-phosphate dehydrogenase was used as a loading control. (B and C) MTT and colony formation assays, respectively, show that growth rates and colony sizes are decreased in TBLR1-silenced cells. DAPI, 4',6-diamidino-2-phenylindole. (D) Representative micrographs (left panel) and quantification (right panel) of 5-bromodeoxy uridine (BrdU)-incorporated breast cancer cells. (E) Flow cytometric analyses showing the percentages of TBLR1-shRNA transfected cells and vector controls at different phases of the cell cycle. The data are representative of three independent experiments. All values are given as the mean ± SD of three independent experiments; *P <0.05.

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Breast Cancer Research

ISSN: 1465-542X

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