Figure 3

Transducin (β)-like 1 X-linked receptor 1 (TBLR1) overexpression promotes cell proliferation and G1-S phase transition in breast cancer cells. (A) Western blotting shows the levels of TBLR1 protein in TBLR1-overexpressing breast cancer cell lines; glyceraldehyde-3-phosphate dehydrogenase was used as a loading control. (B and C) MTT and colony formation assays, respectively, show that growth rates and colony sizes are increased in TBLR1-overexpressing breast cancer cells. (D) Representative micrographs (left panel) and quantification (right panel) of 5-bromodeoxy uridine (BrdU)-incorporated breast cancer cells. DAPI, 4',6-diamidino-2-phenylindole. (E) Flow cytometric analyses showing the percentages of cells of TBLR1-overexpressing cells and vector control cells at different phases of the cell-cycle. The data are representative of three independent experiments. All values are given as the mean ± SD of three independent experiments; *P <0.05.