Figure 4

Inhibition of vascular endothelial growth factor C sensitizes 66 cl4 mammary carcinoma cells to oxidative stress and to chemotherapeutic agents. (A) Inhibition of vascular endothelial growth factor C (VEGF-C) in 66 cl4 cells decreases colony formation in the presence of doxorubicin (Dox). (B) Inhibition of VEGF-C in 66 cl4 cells decreases colony formation in the presence of phenethyl isothiocyanate (PEITC). Representative quantification (of three independent experiments) is shown for the number of colonies formed in 66 cl4-scram and VEGF-C KD1/KD2 cells (top). Representative pictures of colonies formed in 66 cl4-scram and VEGF-C KD cells treated with doxorubicin (3 μM) or PEITC (1 μM) or the corresponding vehicle control (Ctrl; bottom). (C) Representative flow data show the staining of live (calcein AM dye) and dead (ethidium homodimer (EthD) 1 dye) cell populations in 66 cl4-scram and VEGF-C KD cells treated with control (H₂O) or H₂O₂ (1 mM) (left). Quantification of dead cells from 66 cl4-scram and 66 cl4-VEGF-C KD1/KD2 cells treated with control and put under oxidative stress (combination of three independent experiments).