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Figure 2 | Breast Cancer Research

Figure 2

From: Vascular endothelial growth factor C promotes breast cancer progression via a novel antioxidant mechanism that involves regulation of superoxide dismutase 3

Figure 2

Vascular endothelial growth factor C-knockdown decreases the aldehyde dehydrogenase-positive tumor-initiating cell population. (A) Expression of vascular endothelial growth factor C (VEGF-C) mRNA in bulk tumors or tumorspheres formed from cells of primary breast tumors. Gene expression levels were obtained from public microarray data sets [31]. (B) Expression of VEGF-C in attached parental cells or tumorspheres formed by growing 66 cl4 cells in serum-free suspension conditions. Pictures of attached cells and tumorspheres are shown. (C) Representative flow cytometric data in an ALDEFLUOR assay. Diethylaminobenzaldehyde (DEAB) is a specific inhibitor of aldehyde dehydrogenase (ALDH), which was used as a gating control. (D) Quantification of the ALDH-positive population in scramble control (scr) and VEGF-C KD lines from 66 cl4 and MDA-MB-231 cells. Data were combined from three independent experiments using one scramble KD and two VEGF-C KD (KD 1 and 2) cell lines for both 66 cl4 and MDA-MB-231 cells.

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Breast Cancer Research

ISSN: 1465-542X

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