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Figure 3 | Breast Cancer Research

Figure 3

From: RETRACTED ARTICLE: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis

Figure 3

Nuclear factors of activated T cells 5 (NFAT5)/S100A4 expedites epithelial-mesenchymal transition (EMT) and invasion of breast cancer cells. (A) Transwell assay for the invasiveness of cells transfected with siRNAs targeting the indicated genes. (B) qRT-PCR assays for the expression of metastasis-related genes in MDA-MB-231 cells transfected with indicated siRNAs. Relative mRNA levels compared with untransfected cells were plotted. NC, scrambled negative control of siS100A4. (C) Microscopy of MDA-MB-231 cells showing suppressed spreading and increased intercellular contact upon introduction of S100A4-targeting siRNA. (D to F) Fluorescent microscopy (D), western blotting (E) and qRT-PCR (F) assays of MDA-MB-231 cells after transfection with siRNAs targeting the indicated genes. (G) Representative photographs of immunohistochemical staining of breast cancers in clinical stages I to IV. Relative levels compared with untransfected cells were plotted for qRT-PCR assays in (B) and (F). All data are representative images or represent the mean ± SD of three replicates. *P <0.05.

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