Figure 7From: Elevated DDX21 regulates c-Jun activity and rRNA processing in human breast cancersNucleolar DDX21 promotes rRNA processing in breast cancer cells. (A) HCC1806 cells were infected with shSCR or shRNA-DDX21, two days postinfection, cells were pulsed with [3H-methyl] methionine and chased for 1 hour. Total RNA was extracted and analyzed for 47S, 32S, 28S and 18S rRNA levels. Methylene blue-stained membrane is also shown at the bottom. (B) HCC1806 cells were infected with shSCR or shRNA-DDX21, two days postinfection, cells were pulsed with [3H-methyl] methionine and chased for 0, 25 and 50 min. Total RNA was extracted and analyzed for 47S, 32S, 28S and 18S rRNA levels. Methylene blue-stained membrane is also shown at the bottom.Back to article page