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Figure 4 | Breast Cancer Research

Figure 4

From: Elevated DDX21 regulates c-Jun activity and rRNA processing in human breast cancers

Figure 4

DDX21 is important for the tumorigenicity of breast cancer cells in vitro and in vivo . (A) T47D, MCF-7 and SKBR3 cells were transduced with either shSCR or shRNA-DDX21 lentiviruses. DDX21 levels were detected by western blot analysis after lentiviral knockdown. Tubulin was used as a loading control. (B) Above-mentioned cells were also subjected to soft agar analysis after plating 1 × 104cells in each 60 mm dish. Typical image is shown after culturing for two weeks. (C) Quantitation of colony numbers in the soft agar assay is graphed; error bars were taken from triplicates. (D) A group of five SCID mice were injected at the mammary gland on both sides with MDA-MB-231-luciferase reporter cells transduced with either shScrambled or with shDDX21 (50,000 cells per injection). Mice were allowed to recover from the surgery and bioluminescence imaging was performed from two weeks to nine weeks. A representative mouse from each group is shown at the indicated time points. Data presented show the mean × standard deviation from five different mice in each group. SCID, severe combined immunodeficiency.

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