Figure 3From: Identification of chemokine receptors as potential modulators of endocrine resistance in oestrogen receptor–positive breast cancersAnalysis of CXCR4 and CXCR7 in cell lines and clinical data on association with recurrence of oestrogen receptor–positive breast cancer. wt-MCF7, MCF7-LTED, wt-SUM44 and SUM44-LTED cells were transfected with siCXCR4 (A) or siCXCR7 (B) versus sicontrol × exogenous oestradiol (E2) (1 nM). Cells were cultured for 6 days. Cell survival was measured using CellTiter-Glo. The data are expressed as fold changes relative to sicontrol. Each treatment was carried out with eight replicates. The data shown are representative of a minimum of five independent experiments. Bars represent × standard error of the mean (SEM). *P <0.05, **P <0.01, ***P <0.001. (C) Kaplan-Meier analysis of the influence of CXCR4 and CXCR7 on relapse-free survival (RFS) in patients with oestrogen receptor–positive (ER+) breast cancer (BC), who were either treatment-na–ve or treated with tamoxifen, whose data were derived from publicly available clinical BC data sets collected over 12 years [13]. Data were stratified by the highest quartile versus the rest. CXCR, Chemokine C-X-C receptor; DCC, Dextran-coated charcoal; LTED, Long-term oestrogen deprivation; si, Small interfering; wt, Wild type.Back to article page