Cytoplasmic interleukin-like epithelial-to-mesenchymal transition inducer localization correlates with high tumor-cell associated urokinase plasminogen activator receptor expression, and a combined ILEI-uPAR marker analysis shows enhanced prognostic power in human breast cancer. (A) Immunohistochemistry for interleukin-like epithelial-to-mesenchymal transition inducer (ILEI) localization (granular or cytoplasmic, upper images) and urokinase plasminogen activator receptor (uPAR) expression (low or high, lower images) on representative samples from two human breast cancer tissue arrays. ILEI/uPAR signal is in purple, and genomic DNA is in blue (counterstained with hematoxylin and eosin). Scale bar, 50 μm. (B) Distribution of tumor-cell associated uPAR protein expression levels in 88 patients with different subcellular ILEI localizations. (C) Evaluation of ILEI localization (left panel), tumor-cell associated uPAR expression (middle panel) and a combined analysis of the two markers (right panel) for metastasis-free survival depicted in Kaplan-Meier plots.