Aprotinin suppresses increased tumor growth and lung colonization of wild-type interleukin-like epithelial-to-mesenchymal transition inducer–overexpressing EpC40 cells. Analysis of primary tumor growth and lung colonization capacity of EpC40 cells and derivatives in aprotinin (Ap)-treated mice was performed as described in the Figure 2 legend (n = 10 per group). Five mice in each group were administered with 4,000 kallikrein inactivator units of aprotinin daily. Error bars show mean ± SEM. (A) Tumor growth rate. (B) Tumor masses. (C) Lung colonization assay. Moribund mice were killed and analyzed for lung metastases. The experiment was terminated 80 days after tumor cell injection. The frequency of survival is represented in two separate Kaplan-Meier plots, with the upper showing untreated mice and the lower aprotinin-treated mice.