Immunohistochemical intensity of nuclear expression for p-Akt correlates with cytoplasmic staining for CIP2A. Upper panel shows representative immunohistochemical patterns of p-Akt in estrogen receptor (ER)–negative human breast cancer tissues showing strong nuclear expression (A), moderate nuclear expression (B), mild nuclear expression (C) and negative nuclear expression (D). No cytoplasmic expression of p-Akt was noted. Lower panel shows representative immunohistochemical patterns of cancerous inhibitor of protein phosphatase 2A (CIP2A) in cancer cells showing strong cytoplasmic expression (A), moderate cytoplasmic expression (B), mild cytoplasmic expression (C), negative cytoplasmic expression (D). Original magnification in upper and lower panels, 200×. (E) Left: CIP2A and p-Akt expression in tumor samples from 123 patients with ER-negative breast cancers (see Tables 1 and 2 for details). Right: Progression-free survival curves for patients with high CIP2A expression (n = 20) and patients with moderate to low or negative CIP2A expression (n = 103). Curves were generated by using the Kaplan-Meier method and compared by performing a logrank test.