Skip to main content
Figure 6 | Breast Cancer Research

Figure 6

From: Trefoil factor 3 promotes metastatic seeding and predicts poor survival outcome of patients with mammary carcinoma

Figure 6

Forced expression of TFF3 in MCF7 cells enhanced phosphorylation of c-SRC that subsequently increased STAT3 activity to promote invasion. (A) Western blot analysis was used to assess the levels of p-c-SRC and c-SRC in MCF7 cells with either forced or depleted expression of TFF3 as described in Methods. (B) Western blot analysis was used to assess the levels of pSTAT3, STAT3, and CDH1 in MCF7 cells with forced expression of TFF3 on exposure to PP1 (5 μM), PP2 (2 μM) or PP3 (50 μM) as described in Methods. (C) CDH1 promoter activity in MCF7 cells with either forced or depleted expression of TFF3, on exposure to PP1 (5 μM), PP2 (2 μM) or PP3 (50 μM). The luciferase assay was performed as described in Methods. (D) α2-M promoter activity in MCF7 cells with either forced or depleted expression of TFF3, on exposure to PP1 (5 μM), PP2 (2 μM), or PP3 (50 μM). The luciferase assay was performed as described in Methods. (E). Invasive capacity of MCF7 cells with either forced or depleted expression of TFF3, on exposure to PP1 (5 μM), PP2 (2 μM) or PP3 (50 μM); and/or in combination with transiently transfected STAT3 CA. Cell invasion was evaluated using a Transwell assay. Statistical significance was assessed by using an unpaired two-tailed Student's t test (P <0.05 was considered as significant) using GraphPad Prism 5. Columns are the mean of triplicate experiments; bars, ± SD. **P <0.001, *P <0.05.

Back to article page