Nestin silencing inhibits breast CSC migration by modulating the expression of EMT-related genes (A). The migration of Nestin-si and Nestin-c breast CSC. Nestin-si and Nestin-c breast CSC were incubated in the upper chambers of transwell plates at 37 °C for 1.5 h. The migrated cells were stained with crystal violet and counted. Results are presented as mean ± SD of the numbers of migrated cells per group of cells. (B) and (C). The relative levels of EMT-related protein expression in CSC. Nestin-si and Nestin-c breast CSC were incubated for 48 h, and the relative levels of EMT-related proteins to β-actin were determined by western blot analysis. Data shown are representative images and expressed as the mean ± SD of each target protein in each type of cells from six separate experiments. *P <0.05 vs. the Nestin-c. CSC, cancer stem cell; EMT, epithelial-mesenchymal transition; SD, standard deviation.