Nestin expression is associated with poor survival in patients with triple-negative breast cancer. Nestin expression in 150 surgically resected breast tissue samples from patients with triple-negative breast cancer was examined by immunohistochemistry. Survival was estimated using the Kaplan-Meier method, and the association between survival and Nestin expression was analyzed using the log-rank test. Data are representative images (400× magnification) and the cumulative survival of 150 patients. (A) Positive anti-Nestin staining in myoepithelial cells in the surrounding nontumor areas; (B, C) negative and positive anti-Nestin staining in breast tumors (white arrow indicates positive cytoplasmic staining). (D) Cumulative survival of patients with triple-negative breast cancer.