Figure 4From: PIK3CA mutations in androgen receptor-positive triple negative breast cancer confer sensitivity to the combination of PI3K and androgen receptor inhibitorsGenetic targeting of androgen receptor (AR) is additive in combination with PI3K inhibition. (A) Immunoblot shows AR protein levels at 72 h following transduction with two shRNAs targeting AR (shAR-1 and shAR-2) compared to transduction with nontargeting shRNA (shNT) in the indicated AR + triple-negative breast cancer (TNBC) cell lines. Actin levels are shown for the loading control. (B) Line graphs display relative viability of AR + cell lines transduced with nontargeting (shNT) or shRNAs targeting AR (shAR-1 and shAR-2) after 72 h treatment with increasing concentrations of the pan-PI3K inhibitor GDC-0941 (top) or the dual PI3K/mTOR inhibitor GDC-0980. Error bars represent SD for three independent infections.Back to article page