Table 1 The CLDN1, CLDN3, CLDN4 and GAPDH primer sequences, position and product size
Primer | Forward | Reverse | Product size (bp) | GI number | ||||
|---|---|---|---|---|---|---|---|---|
Sequence 5'–3' | Size (bp) | Sequence 5'–3' | Size (bp) | |||||
CLDN1 | ttcgtacctggcattgactgg | 378–399 | 22 | ttc gta cct ggc att gac tgg | 470–490 | 21 | 112 | 4559277 |
CLDN3 | ctgctctgctgctcgtgtcc | 732–751 | 20 | ttagacgtagtccttgcggtcgtag | 836–860 | 25 | 128 | 21536298 |
CLDN4 | ggctgctttgctgcaactgtc | 741–761 | 21 | gagccgtggcaccttacacg | 829–848 | 20 | 107 | 14790131 |
GAPDH | gaagatggtgatgggatttc | 81–98 | 20 | gaaggtgaaggtcggagt | 287–306 | 18 | 225 | 7669491 |