Table 2 Primary antibodies used for immunohistochemistry
Antibody | Dilution | Source | |
|---|---|---|---|
Phospho-Akt (1/2/3) Serin 473 | Polyclonal | 1:200 | Cell Signaling Technology |
Phospho-Akt (1/2/3) Serin 473 | Polyclonal | 1:2000 | Santa Cruz Biotechnology |
Focal adhesion kinase (C-20) | Polyclonal | 1:100 | Santa Cruz Biotechnology |
Phospho-focal adhesion kinase tyrosine 861 | Polyclonal | 1:50 | Santa Cruz Biotechnology |
Phospho-Src tyrosine 215 | Polyclonal | 1:200 | Sigma-Aldrich |
Phospho-Src tyrosine 416 | Polyclonal | 1:200 | Cell Signaling Technology |
Phospho p44/42 Map kinase (Erk1/2) threonine 202;tyrosine 204 | Monoclonal | 1:100 | Cell Signaling Technology |
Estrogen receptor | Monoclonal | 1:25 | DAKO |