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Figure 4 | Breast Cancer Research

Figure 4

From: Knockdown of c-Myc expression by RNAi inhibits MCF-7 breast tumor cells growth in vitro and in vivo

Figure 4

Knockdown of c-Myc by RNAi reduces colony formation in soft agar. MCF-7 cells were transfected with pSilencer–c-Myc or pSilencer as controls, and seeded in 0.35% agarose containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum. The colony numbers were counted 2 weeks later. (a) Representative wells demonstrating the total number of colonies formed by MCF-7 transfected with the indicated plasmids. (b) The numbers of colonies of pSilencer–c-Myc-treated cells standardized against the control cells (set at 100%). The data shown are means and SD from two independent triplicate experiments. The difference between treatments is statistically significant (P < 0.001).

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