Figure 9
From: Membrane estrogen receptor-α levels predict estrogen-induced ERK1/2 activation in MCF-7 cells
Pulsed effect of 17β-estradiol (E2) and conjugated E2 on cell proliferation. MCF-7 membrane estrogen receptor (mER)-α-enriched (mERhigh) MCF-7 cells were treated for 5 days (bar 1) or 10 min with 1 pmol/l E2 (bar 2) or E2-peroxidase containing the equivalent of 1 pmol/l E2 (bar 3). In bar 4, pretreatment with U0126 (40 μmol/l) for 15 min was followed by 10 min treatment with E2. Bar 5 shows blockage of mER-α by incubating the cells for 1 hour at room temperature with 1 μg/ml of a ligand binding domain-specific antibody and the subsequent effect of 10 min incubation with E2. These experiments were repeated three times, each with 40 well replicates; values are expressed as mean ± standard error. The asterisks indicate significant differences from the controls (C – ethanol vehicle, inhibitor, or antibody treatment alone).