Figure 8From: Membrane estrogen receptor-α levels predict estrogen-induced ERK1/2 activation in MCF-7 cellsDose-dependent 17β-estradiol (E2) activation of extracellular signal-regulated kinase (ERK)1/2 in membrane estrogen receptor (mER)-α-enriched (mERhigh) versus mER-α-depleted (mERlow) MCF-7 cells. Cells were incubated with different E2 concentrations at peak activation times: (a) 10 min in the case of mERhigh and (b) 6 min in the case of mERlow. The experiments were repeated three times, each with 16 replicates; values are expressed as mean ± standard error. The asterisks indicate significant differences from the vehicle controls (C).Back to article page