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Figure 11 | Breast Cancer Research

Figure 11

From: Membrane estrogen receptor-α levels predict estrogen-induced ERK1/2 activation in MCF-7 cells

Figure 11

Effects of ICI182,780 (ICI), ICI + 17β-estradiol (E2), and 17α-estradiol on extracellular signal-regulated kinase (ERK)1/2 activation in membrane estrogen receptor-α-enriched (mERhigh) MCF-7 cells. (a) Time course of ERK1/2 activation by 1 μmol/l ICI. (b) Pretreatment with ICI (1 μmol/l) for 30 min followed by 1 pmol/l E2 stimulation in the continued presence of ICI. (c) ICI (1 μmol/l) and E2 (1 pmol/l) applied simultaneously. (d) Time course of 17α-estradiol (10 nmol/l) activation of ERK1/2. All experiments were repeated at least three times with 24 well replicates/experiment; the averaged values ± standard error are presented. The asterisks indicate significant differences from vehicle controls.

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