Figure 10
From: Membrane estrogen receptor-α levels predict estrogen-induced ERK1/2 activation in MCF-7 cells
Effects of phosphatase inhibitors on extracellular signal-regulated kinase (ERK)1/2 dephosphorylation. Cells were pretreated for 1 hour with different phosphatase inhibitors: 50 nmol/l okadaic acid (OA) or 3 μmol/l cyclosporin A (CyclA). Then, ERK1/2 phosphorylation was determined after 1 pmol/l E2 stimulation for (a, b) 20 min or (c) 60 min. Black bars are membrane estrogen receptor (mER)-α-enriched (mERhigh) MCF-7 cells; open bars are mER-α-depleted (mERlow) MCF-7 cells. The data are averages of two or three experiments, each with 24 replicates; values are expressed as mean ± standard error. The asterisks indicate significant differences from the vehicle control-treated samples with corresponding inhibitor included.