Figure 1
From: Membrane estrogen receptor-α levels predict estrogen-induced ERK1/2 activation in MCF-7 cells
Fluorescence immunocytochemical detection of membrane estrogen receptor (mER)-α in nonpermeabilized MCF-7 cells. Cells were fixed with 2% pararaformaldehyde/0.1% glutaraldehyde, probed with C-542 carboxyl-terminal estrogen receptor-α antibody, and visualized with a biotinylated secondary antibody–avidin conjugated alkaline phosphatase fluorescent Vector red product. Fluorescence images were photographed using the FITC filter and 100 × magnification. (a) mER-α-enriched (mERhigh) MCF-7 cells; the arrows indicate some of the punctate staining. (b) mER-α-depleted (mERlow) MCF-7 cells have no staining. The bar in panel b represents 10 μm.