Skip to main content

Table 3 Relative gene expression in ZR-75-1-derived cell lines

From: Breast cancer oestrogen independence mediated by BCAR1 or BCAR3 genes is transmitted through mechanisms distinct from the oestrogen receptor signalling pathway or the epidermal growth factor receptor signalling pathway

 

ZR-75-1 cells

BCAR1-transfected cells

BCAR3-transfected cells

EGFR-transfected cells

Addition...a

None

ICI

Oestrogen

None

Oestrogen

None

Oestrogen

None

EGF

  

6 h

6 h

30 h

96 h

Cont.

 

6 h

30 h

96 h

 

6 h

30 h

96 h

 

3 days

7 days

Gene

3 (4)b

1 (1)

2 (4)

1 (1)

1 (3)

1 (3)

2 (6)

2 (3)

1 (1)

1 (1)

2 (4)

2 (3)

1 (1)

1 (1)

1 (3)

1 (2)

1 (4)

HMBS

(23.1) 1c

0.58

0.79

0.42

0.49

1.22

0.93

0.75

0.51

0.93

0.59

0.56

0.41

0.64

0.93

0.80

0.99

FMOD d

(29.4) 1

1.60

1.47

0.79

1.04

0.95

0.58

0.83

0.71

0.81

0.95

1.09

1.00

1.59

1.02

0.94

0.54

PGK1 d

(22.3) 1

1.33

1.02

1.08

1.18

1.18

1.41

0.94

1.07

1.61

1.46

2.10

0.97

2.11

0.70

2.23

0.94

CDKN1A d

(25.8) 1

0.60

0.72

0.47

0.66

2.03

1.45

1.24

0.61

1.34

1.19

1.37

0.81

1.78

1.26

2.11

3.60

MYC

(23.3) 1

0.55

1.21

0.41

0.90

0.97

0.72

0.98

0.47

0.74

0.47

0.60

0.51

0.76

1.00

0.44

0.26

CTSD

(18.7) 1

0.68

2.13

2.25

1.94

3.95

0.91

1.89

1.87

3.02

0.77

1.43

1.90

4.21

1.78

2.38

1.95

TFF1

(21.2) 1

0.56

2.07

9.28

20.45

36.67

0.99

2.38

13.37

32.60

0.94

1.35

9.30

21.37

4.71

5.77

2.64

PGR

(24.6) 1

0.71

9.08

9.24

12.00

7.52

0.83

7.24

9.29

7.65

0.38

2.22

3.55

5.62

0.56

0.32

0.05

PDKZ1 d

(26.4) 1

0.92

5.26

14.75

10.12

16.66

0.64

1.85

11.86

11.81

0.52

1.93

7.26

9.10

1.17

0.08

0.03

TFF3 d

(23.0) 1

0.50

1.03

2.64

6.19

11.56

0.84

1.30

2.60

7.29

1.21

1.23

2.22

4.02

1.09

0.79

0.10

MGP d

(22.8) 1

0.83

1.09

1.54

2.99

1.34

0.93

1.00

3.12

3.15

0.89

0.78

2.14

1.11

0.42

0.86

0.23

IGFBP5

(17.6) 1

0.80

0.92

0.24

0.45

0.17

0.91

0.74

0.27

0.49

0.33

0.30

0.22

0.28

1.46

0.04

0.01

APOD d

(21.5) 1

0.96

0.89

0.53

0.25

0.09

0.37

0.36

0.18

0.08

0.60

0.59

0.36

0.27

0.32

0.02

0.04

CSTA d

(27.7) 1

0.57

0.86

0.08

0.06

0.06

0.74

0.53

0.08

0.09

2.95

2.86

0.88

0.44

0.04

0.01

0.02

HER2/Neu

(20.4) 1

0.77

0.75

0.18

0.29

0.39

0.73

0.48

0.20

0.25

0.68

0.53

0.29

0.37

1.16

0.51

0.49

IL1R1 d

(27.5) 1

0.76

0.50

0.10

0.09

0.04

1.54

0.54

0.07

0.15

0.64

0.40

0.14

0.23

0.84

0.12

0.21

ERα

(20.2) 1

0.54

0.86

0.36

0.41

0.27

0.50

0.55

0.31

0.29

0.35

0.39

0.28

0.33

0.79

0.12

0.07

ERβ

(32.7) 1

1.21

1.93

0.05

1.57

1.62

1.61

1.40

0.63

0.60

0.84

1.78

0.42

3.19

1.58

1.08

1.89

BCAR3

(24.2) 1

0.74

0.51

0.40

0.46

0.45

0.64

0.25

0.14

0.23

18.30

14.36

13.19

24.78

0.35

0.60

0.69

BCAR1

(24.8) 1

0.93

1.17

0.71

0.69

1.31

55.96

31.90

13.67

22.12

0.80

1.01

0.58

0.36

1.01

1.13

1.07

PSAT1 d

(35.6) 1

0.86

0.67

0.40

0.97

2.64

<0.01

<0.01

<0.01

<0.01

0.87

1.16

0.84

0.76

1.65

3.36

3.64

EGFR

(35.0) 1

0.25

0.84

1.62

1.75

1.27

1.47

2.56

2.31

1.25

2.24

2.15

2.75

4.13

8126

11045

10582

  1. All expression levels have been normalised for HPRT1 levels and are presented relative to non-stimulated ZR-75-1 cells. The gene names are defined n Additional file 3.
  2. aThe nature and duration of the culture additions (ICI, oestrogen antagonist ICI 164,384).
  3. bThe number of biological replicates and total number of analyses (in parentheses).
  4. cGene expression level was set at 1.0 for non-stimulated ZR-75-1 cells and the detection threshold cycle (Ct) value for each gene is shown in parentheses (23 for HPRT1).
  5. dQuantitative polymerase chain reaction with assay on demand; others with SYBR green.
Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us