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Table 1 Hybridisation experiments

From: Breast cancer oestrogen independence mediated by BCAR1 or BCAR3 genes is transmitted through mechanisms distinct from the oestrogen receptor signalling pathway or the epidermal growth factor receptor signalling pathway

RNA no.

Microarray hybridisations

1

BCAR3 (R/BCS)

BCAR3 (E2 6 h)

BCAR3 (R/BCS)

ZR-75-1 (E2 6 h)

ZR-75-1 (E2 7 d)

ZR-75-1 (E2 7 d)

ZR-75-1 (E2 7 d)

ZR-75-1 (E2 7 d)

2

BCAR1 (R/BCS)

BCAR3 (R/BCS)

ZR-75-1 (R/BCS)

ZR-75-1 (R/BCS)

ZR-75-1 (R/BCS)

BCAR1 (R/BCS)

BCAR3 (R/BCS)

ZR/EGFR (EGF 7 d)

  1. RNA samples were prepared from cell lines pretreated for 5 days in RPMI 1640 medium supplemented with 10% bovine calf serum (R/BCS) and subsequently cultured for the indicated durations in R/BCS medium supplemented with oestrogen (E2), epidermal growth factor (EGF) or R/BCS medium without further additions. All hybridisations were performed in duplicate with swapping of the dyes.
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Breast Cancer Research

ISSN: 1465-542X

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