Table 4 Insulin-like growth factor (IGF) II ELISA for normal breast-associated fibroblasts (NAF), carcinoma-associated fibroblasts (CAF) and MCF10AT cell monocultures and co-cultures in a three-dimensional in vitro model
From: Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture
Cell type | IGF II level (normalized to total protein) | |||
|---|---|---|---|---|
| Â | Monoculture | Co-culture with MCF10AT cells | ||
|  | Level (ng/μg) | Mean (ng/μg) | Level (ng/μg) | Mean (ng/μg) |
NAF | Â | Â | Â | Â |
   NAF-1 | 49.25 |  | 9.68 |  |
   NAF-2 | 45.00 |  | 10.22 |  |
   NAF-3 | 68.14 | 54.13 | 10.27 | 10.06 |
CAF | Â | Â | Â | Â |
   CAF-1 | 70.64 |  | 10.01 |  |
   CAF-2 | 52.37 |  | 10.88 |  |
   CAF-3 | 52.96 | 58.65 | 9.96 | 10.28 |
Breast epithelial cells | ||||
MCF10AT | 9.98 | Â | Â | Â |