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Table 4 Insulin-like growth factor (IGF) II ELISA for normal breast-associated fibroblasts (NAF), carcinoma-associated fibroblasts (CAF) and MCF10AT cell monocultures and co-cultures in a three-dimensional in vitro model

From: Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture

Cell type IGF II level (normalized to total protein)
  Monoculture Co-culture with MCF10AT cells
  Level (ng/μg) Mean (ng/μg) Level (ng/μg) Mean (ng/μg)
   NAF-1 49.25   9.68  
   NAF-2 45.00   10.22  
   NAF-3 68.14 54.13 10.27 10.06
   CAF-1 70.64   10.01  
   CAF-2 52.37   10.88  
   CAF-3 52.96 58.65 9.96 10.28
Breast epithelial cells
MCF10AT 9.98