Table 4 Insulin-like growth factor (IGF) II ELISA for normal breast-associated fibroblasts (NAF), carcinoma-associated fibroblasts (CAF) and MCF10AT cell monocultures and co-cultures in a three-dimensional in vitro model
From: Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture
| Cell type | IGF II level (normalized to total protein) | |||
|---|---|---|---|---|
| Monoculture | Co-culture with MCF10AT cells | |||
| Level (ng/μg) | Mean (ng/μg) | Level (ng/μg) | Mean (ng/μg) | |
| NAF | ||||
| NAF-1 | 49.25 | 9.68 | ||
| NAF-2 | 45.00 | 10.22 | ||
| NAF-3 | 68.14 | 54.13 | 10.27 | 10.06 |
| CAF | ||||
| CAF-1 | 70.64 | 10.01 | ||
| CAF-2 | 52.37 | 10.88 | ||
| CAF-3 | 52.96 | 58.65 | 9.96 | 10.28 |
| Breast epithelial cells | ||||
| MCF10AT | 9.98 | |||