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Table 3 Insulin-like growth factor (IGF) II ELISA for normal breast-associated fibroblasts (NAF), carcinoma-associated fibroblasts (CAF), MCF10A cells and MCF10AT cells in monolayer cultures

From: Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture

Cell type

IGF II level (normalized to total protein)

 

Level (ng/μg)

Mean (ng/μg)

NAF

  

   NAF-1

11.63

 

   NAF-2

6.87

 

   NAF-3

10.31

 

   NAF-4

7.21

 

   NAF-5

2.92

7.79

CAF

  

   CAF-1

11.80

 

   CAF-2

10.58

 

   CAF-3

10.59

 

   CAF-4

11.27

 

   CAF-5

10.21

 

   CAF-6

14.07

11.42

Breast epithelial cells

  

   MCF10A

5.56

 

   MCF10AT

4.17

4.87