Figure 3
From: Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture
Distribution and relative quantities of fibroblasts and epithelial cells in a normal terminal duct-lobular unit, hyperplasia and ductal carcinoma in situ (DCIS). (a) A terminal duct-lobular unit with epithelial cells (arrowheads) arranged in acini and intralobular terminal ducts separated by stroma-containing fibroblasts (arrows) in a ratio of epithelial cells to fibroblasts (E:F) of 2.7:1. (b) Ductal hyperplasia with a proliferation of epithelial cells (arrowheads) filling and expanding terminal ducts separated by reactive stroma including fibroblasts (arrows) in an E:F of 3.3:1. (c) High-grade DCIS, with epithelial cells (arrowheads) demonstrating markedly atypical nuclei, involving terminal ducts separated by reactive stroma including fibroblasts (arrows) in an E:F of 2:1 (200 × magnification; scale bar, 50 μm).