Proteotypic classification of spontaneous and transgenic mammary neoplasms

Mikaelian, Igor; Blades, Natalie; Churchill, Gary A; Fancher, Karen; Knowles, Barbara B; Eppig, Janan T; Sundberg, John P

doi:10.1186/bcr930

Breast Cancer Research

Table 1 Proteotypic Classification of Spontaneous and Transgenic Mammary Neoplasms Based on Terminal Differentiation Markers^a.

From: Proteotypic classification of spontaneous and transgenic mammary neoplasms

Type	Strain/ Transgene ^b	Main pattern^c	Myoepithelial differentiation^d	EMT (%)^e	K1	K5	K6	K8/18	K10	K14	K17	FLG	IVL	LOR	THH	SMA	VIM
Simple	FVB-Myc	Glandular	6/9	4/9 (<1%)		+	+	+		++	+
	FVB-Notch4	Glandular	0/6	0				+		+	+
	C57BL6/J-Tag	Glandular	1/5	0				+		+	+
	BALB/cJ (mostly)	Papillary, 1 cell thick	2/6	0		+	+	++		++	++
	C57BL6/J-Tag	Papillary	0/4	0				+		+	+
	FVB-Myc	Solid	1/4	3/4 (<1%)				+		+	+
	FVB-Neu	Solid	0/10	0
Complex	FVB and B6D2-Tgfa	Macrocyst	6/8	0		+	+	+++		+	+					+
	FVB and B6D2-Tgfa	Lactation plaque	3/3	0		++	+	+++		++	+					+
	BALB/cJ	Adenomyoepithelioma	5/5	0	+	+++	++	++		+++	++
	BALB/cJ (mostly)	Adenoacanthoma	7/7	0	++	++	++	++	++	+++	+++	++	++	+	+	+
	C3H/HeJ	Microacinar	8/8	0		++	+	++		++	+
	BALB/cJ, C3H/HeJ	Papillary, 2 cells thick	2/2	0		++		++	+	++	++					+++
	C3H/HeJ	Type P tumor	9/9	0		++	+	++		++	++		+			+++
	FVB-Wnt1	Type P tumor	6/6	0		++	++	+++		++	++	+	+			++
	B6SJL-Wnt1	Type P tumor	7/7	0		+	+	+		++	+					++
EMT	C57BL6/J-Tag	Glandular with EMT	2/3	3/3 (6%)				+++		+++	++					++	+
	FVB-Myc	Glandular with EMT	4/6	6/6 (20%)		+	+	++		++	++					+	+
	PL/J and SJL/J	Glandular with EMT	4/4	4/4 (38%)		++	+	+++		++	++		+			++	++
	FVB-Hras	Solid with EMT	3/4	3/4 (<1%)		+		++		+	+						+

^adata are coded, with "+++" representing highest grades; the intensity of immunolabeling was scored 0–3 (immunolabeling levels: 0 = none; 1 = weak; 2 = moderate; 3 = intense); the grade for each cell type was the product of average labeling intensity and the relative percentage of cells (0: 0; ≤ 1%: 1; 2–5%: 2; 6–25%: 3; 26–50%: 4; >50%: 5) labeled at the predominant intensity; outcome for each neoplasm was the sum of the grades for each cell type and the data presented are the average for each group; white boxes indicate an average grade <1; "+" indicates an average grade ≥ 1 and <5; "++" indicates an average grade ≥ 5 and <10; "+++" indicates an average grade ≥ 10; EMT, epithelial to mesenchymal transition; FLG, filaggrin; IVL, involucrin; K1, keratin 1; K5, keratin 5; K6, keratin 6; K8/18, keratins 8 and 18; K10, keratin 10; K17, keratin 17; LOR, loricrin; THH, trichohyalin; SMA, α-smooth muscle actin; VIM, vimentin.
^bAbsence of information for the transgene indicates that the tumor arose in a non-transgenic mouse.
^cBased on the recommendations of the Annapolis meeting [17–19].
^dTumors with myoepithelial differentiation/tumors examined; carcinomas with minimal myoepithelial differentiation arising in mice transgenic for Myc were categorized as "simple carcinomas" because myoepithelial differentiation was interpreted as evidence of early EMT.
^eTumors with EMT/ tumors examined; the data in parenthesis indicate the average percentage of the tumors comprised of neoplastic cells with a mesenchymal phenotype.

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