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Table 1 Proteotypic Classification of Spontaneous and Transgenic Mammary Neoplasms Based on Terminal Differentiation Markersa.

From: Proteotypic classification of spontaneous and transgenic mammary neoplasms

Type Strain/ Transgene b Main patternc Myoepithelial differentiationd EMT (%)e K1 K5 K6 K8/18 K10 K14 K17 FLG IVL LOR THH SMA VIM
Simple FVB-Myc Glandular 6/9 4/9 (<1%)   + + +   ++ +       
  FVB-Notch4 Glandular 0/6 0     +   + +       
  C57BL6/J-Tag Glandular 1/5 0     +   + +       
  BALB/cJ (mostly) Papillary, 1 cell thick 2/6 0   + + ++   ++ ++       
  C57BL6/J-Tag Papillary 0/4 0     +   + +       
  FVB-Myc Solid 1/4 3/4 (<1%)     +   + +       
  FVB-Neu Solid 0/10 0              
Complex FVB and B6D2-Tgfa Macrocyst 6/8 0   + + +++   + +      +  
  FVB and B6D2-Tgfa Lactation plaque 3/3 0   ++ + +++   ++ +      +  
  BALB/cJ Adenomyoepithelioma 5/5 0 + +++ ++ ++   +++ ++       
  BALB/cJ (mostly) Adenoacanthoma 7/7 0 ++ ++ ++ ++ ++ +++ +++ ++ ++ + + +  
  C3H/HeJ Microacinar 8/8 0   ++ + ++   ++ +       
  BALB/cJ, C3H/HeJ Papillary, 2 cells thick 2/2 0   ++   ++ + ++ ++      +++  
  C3H/HeJ Type P tumor 9/9 0   ++ + ++   ++ ++   +    +++  
  FVB-Wnt1 Type P tumor 6/6 0   ++ ++ +++   ++ ++ + +    ++  
  B6SJL-Wnt1 Type P tumor 7/7 0   + + +   ++ +      ++  
EMT C57BL6/J-Tag Glandular with EMT 2/3 3/3 (6%)     +++   +++ ++      ++ +
  FVB-Myc Glandular with EMT 4/6 6/6 (20%)   + + ++   ++ ++      + +
  PL/J and SJL/J Glandular with EMT 4/4 4/4 (38%)   ++ + +++   ++ ++   +    ++ ++
  FVB-Hras Solid with EMT 3/4 3/4 (<1%)   +   ++   + +       +
  1. adata are coded, with "+++" representing highest grades; the intensity of immunolabeling was scored 0–3 (immunolabeling levels: 0 = none; 1 = weak; 2 = moderate; 3 = intense); the grade for each cell type was the product of average labeling intensity and the relative percentage of cells (0: 0; ≤ 1%: 1; 2–5%: 2; 6–25%: 3; 26–50%: 4; >50%: 5) labeled at the predominant intensity; outcome for each neoplasm was the sum of the grades for each cell type and the data presented are the average for each group; white boxes indicate an average grade <1; "+" indicates an average grade ≥ 1 and <5; "++" indicates an average grade ≥ 5 and <10; "+++" indicates an average grade ≥ 10; EMT, epithelial to mesenchymal transition; FLG, filaggrin; IVL, involucrin; K1, keratin 1; K5, keratin 5; K6, keratin 6; K8/18, keratins 8 and 18; K10, keratin 10; K17, keratin 17; LOR, loricrin; THH, trichohyalin; SMA, α-smooth muscle actin; VIM, vimentin.
  2. bAbsence of information for the transgene indicates that the tumor arose in a non-transgenic mouse.
  3. cBased on the recommendations of the Annapolis meeting [17–19].
  4. dTumors with myoepithelial differentiation/tumors examined; carcinomas with minimal myoepithelial differentiation arising in mice transgenic for Myc were categorized as "simple carcinomas" because myoepithelial differentiation was interpreted as evidence of early EMT.
  5. eTumors with EMT/ tumors examined; the data in parenthesis indicate the average percentage of the tumors comprised of neoplastic cells with a mesenchymal phenotype.