Skip to main content
Figure 5 | Breast Cancer Research

Figure 5

From: The Na+–H+exchanger-1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA-MB-435 cells

Figure 5

Effect of dominant negative (dn) RhoA and Rac1 mutants on the intracellular (pHi) dependence of net acid extrusion rate. To examine the mechanism of RhoA and Rac1 modification of the serum deprivation-dependent increase in Na+–H+ exchanger (NHE)1 activity, the intracellular pH (pHi) dependence of NHE1 activity (Δmmol/l H+ i/minute) in MDA-MB-435 cell monolayers was measured. Monolayers were left in fresh whole growth medium (open circles) or serum deprived (closed circles) plus dn-N19RhoA (triangles) or dn-N17Rac1 (squares) for 24 hours. Results from a typical experiment are shown. The plots were computed as described in the Methods section. H+ i, intracellular proton concentration.

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us