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Figure 1 | Breast Cancer Research

Figure 1

From: The Na+–H+exchanger-1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA-MB-435 cells

Figure 1

Efficiency of transfection with Rho family constructs. MDA-MB-435 cell monolayers were transfected with variable concentrations of the pBabe vector containing either the dominant negative (a) N17Rac1 or (b) 17NCdc42 construct. Forty-eight hours later the monolayers were fixed with 4% paraformaldehyde, and the expression of the haemagglutinin (HA) tag visualized by immunofluorescent microscopy with an anti-HA antibody. The percentage of cells expressing HA-tagged N17Rac1 increased in a concentration-dependent manner and reached approximately 90% when the cells were transfected with 10 μg plasmid cDNA.

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