Figure 1
From: Reduced expression of p27 is a novel mechanism of docetaxel resistance in breast cancer cells
Modulation of p27 mRNA expression in docetaxel-resistant breast cancer cells. Total RNA extracted from each cell line was amplified using specific oligonucleotide primer sequences and separated by agarose gel electrophoresis. The p27 and 18S internal control amplified PCR products produced fragment sizes of 253 bp and 489 bp, respectively. Lane 1, DNA size standards; lane 2, MCF-7 docetaxel-resistant subline; lane 3, MCF-7 breast cancer cells; lane 4, MDA-MB-231 breast cancer cells; lane 5, MDA-MB-231 docetaxel-resistant subline.