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Table 1 Evaluation of Her-2/neu gene status using chromogenic in situ hybridization

From: Chromogenic in situ hybridization (CISH): a novel alternative in screening archival breast cancer tissue samples for HER-2/neu status

Amplification Her-2/neu gene status
High-level >10 copies or large cluster of amplicon per nucleus in >50% of cancer cells
Low-level 6–10 copies or small cluster of amplicon per nucleus in >50% of cancer cellsa
None 1–5 copies per nucleus of cancer cellsb
  1. aVerify gene amplification or chromosome 17 aneuploidy with SPOT-Light Chromosome 17 Centromeric Probe. bIf 3–5 copies per nucleus, verify chromosomal aneuploidy with SPOT-Light Chromosome 17 Centromeric Probe.