Figure 4
MSC inhibits TM6 cell migration in vitro. Pretreatment of TM6 cells with Se-methylselenocysteine (MSC) for 1 hour and 24 hours before stimulation with growth factors (GF) and serum decreased the numbers of cells migrating by 44.6% and 57.6%, respectively, to the other end of the membrane in a transwell migration assay. Bar graphs represent the mean of four counts from each of the three separate wells. Error bars are SEM. The inhibition in migration was statistically significant at P < 0.0001 for both pretreatments compared with controls. Only one or two cells were able to migrate in the absence of growth factors.