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Figure 3 | Breast Cancer Research

Figure 3

From: A retinoid X receptor (RXR)-selective retinoid reveals that RXR-α is potentially a therapeutic target in breast cancer cell lines, and that it potentiates antiproliferative and apoptotic responses to peroxisome proliferator-activated receptor ligands

Figure 3

The peroxisome proliferator-activated receptor (PPAR)-α-selective ligand hydroxyeicosatetraenoic acid (HETE) induces apoptosis in human breast cancer cell lines, which is potentiated by AGN194204. (a) The indicated breast cancer cell lines were treated with vehicle (con), 100 μmol/l HETE, 100 nmol/l AGN194204 (AGN), or both compounds simultaneously (HETE+AGN) for 24 hours. The percentage of apoptotic cells was determined by dUTP nick-end labeling (TUNEL) assay and fluorescence microscopy. These experiments were performed three times, with similar results. Error bars indicate the standard error of the mean. (b) The PPAR-α-selective ligand HETE activates the intrinsic apoptotic pathway in human breast cancer cell lines. Whole cell lysates from the indicated breast cancer cell lines treated with 100 μmol/l HETE for up to 24 hours were subjected to western blot, with anti-caspase (casp) and anti-β-actin antibodies shown on the left. Relative expression of each protein was determined using the same membrane. These experiments were performed three times, with similar results. Representative blots are shown.

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Breast Cancer Research

ISSN: 1465-542X

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