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Figure 5 | Breast Cancer Research

Figure 5

From: Investigation of three new mouse mammary tumor cell lines as models for transforming growth factor (TGF)-β and Neu pathway signaling studies: identification of a novel model for TGF-β-induced epithelial-to-mesenchymal transition

Figure 5

Transforming growth factor (TGF)-β1 induces an epithelial-to-mesenchymal transition (EMT) in the BRI-JM01 cell line. (a) Immunofluorescence microscopy demonstrating the altered localization of E-cadherin, N-cadherin and β-catenin, the rearrangement of F-actin and vimentin filaments, and the disappearance of ZO-1 that accompany the TGF-β1 induced EMT (see text for details). (b) Flow cytometry of nonfixed live cells monitoring the presence of E-cadherin at the cell surface of BRI-JM01 cells that were exposed to TGF-β1 for up to 120 hours. Nonspecific isogenic IgG (HA) and cells grown in the absence of TGF-β1 (CTL) were used as a negative and positive control, respectively. (c) Western blot analysis determining the total cellular content of E-cadherin, N-cadherin and β-catenin in BRI-JM01 cells cultured for 24, 72 and 120 hours in the absence (CTL) or presence of TGF-β1 (see text for details). (d) BRI-JM01 cells were treated with TGF-β1 for 48 hours (BRI-JM01T) and passaged several times in the absence of this growth factor. When seeded in Matrigel these 'TGF-β1 primed' BRI-JM01 cells formed larger three-dimensional structures than did the nontreated cells.

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Breast Cancer Research

ISSN: 1465-542X

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