Skip to main content
Figure 4 | Breast Cancer Research

Figure 4

From: Investigation of three new mouse mammary tumor cell lines as models for transforming growth factor (TGF)-β and Neu pathway signaling studies: identification of a novel model for TGF-β-induced epithelial-to-mesenchymal transition

Figure 4

Transforming growth factor (TGF)-β1 induces increased motility in the BRI-JM01 cell line. Endogenous (CTL) and TGF-β1-induced motility of the BRI-JM01, BRI-JM04, and BRI-JM05 cells was determined in a (a) wound-closure assay and a (b) black ink-assay (see text for details). Each experiment was repeated at least twice, with similar results. (c) Particle-free tracks were quantitated (using ImageJ freeware) and expressed as the relative fold ink clearance (± standard error of the mean) of the least motile BRI-JM05 cell line 24 hours after seeding the three cell line in the absence (white bars) or presence of 100 pmol/l TGF-β1 (black bars). BRI-JM01 and BRI-JM04 cells are approximately twice as motile as the BRI-JM05 cells; however, TGF-β1 only stimulates the motility of the BRI-JM01 cells (for details see text).

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us