Figure 2
From: Chromosomal instability and lack of cyclin E regulation in hCdc4 mutant human breast cancer cells
Cyclin E is overexpressed throughout the cell cycle in SUM149PT cells. Two-parameter flow cytometric analysis was performed using fixed and permeabilized (a) MCF10A cells and (b) SUM149PT cells. HE12 cyclin E primary monoclonal antibody and FITC-conjugated secondary antibody were used for cyclin E detection. Cells were incubated with 50 μg/ml propidium iodide (PI) for DNA content analysis.