No germline mutations in the histone acetyltransferase gene EP300 in BRCA1 and BRCA2 negative families with breast cancer and gastric, pancreatic, or colorectal cancer

Campbell, Ian G; Choong, David; Chenevix-Trench, Georgia

doi:10.1186/bcr803

Table 2 Primers used to amplify exons and splice junctions of EP300

From: No germline mutations in the histone acetyltransferase gene EP300 in BRCA1 and BRCA2 negative families with breast cancer and gastric, pancreatic, or colorectal cancer

Exon	Forward¹	Reverse	Product size (base pairs)	Predicted melt temperature (°C)²
1	Atttcctgaggattctggtt	cgcaccgagtagaaaagat	202	57,62
2a	gacctttgtcttttcccttt	accctgatttggtccacta	372	60
2b	aggcaggcttgacttctc	gcacacaaaccaaaactgta	372	60
3	ttgcttattttgttttcttttg	atgccagtttgaaaacaagt	254	52,57
4	agcacattatgactcctaccatt	acagtaaccctgtgggtcc	333	57,62
5	cattaacctgctcttgaaaaa	actggtgagactttaaaactt	210	57
6	ttctcaccagcattaatttgt	tgacacaaccaataccatgt	335	5560
7	tgtctcctgttatttcattttg	tgtaatgtgacccagggtat	161	57
8	aactatttggtgaccccttt	gaatgagacgtgtccacaat	193	54,59
9	aaatgctgacatgatattacagtgg	ccacaacaggttcaatcttgg	212	58
10	tgttacctggtggtagttcc	caaacagaaatataacaaaaacca	266	58,53
11	aatattttgtggggtttgtg	gtacaccggggattctttta	215	58,53
12	aatttcacaaaggcattcag	aggtaaaggccaaagagatt	196	55,60
13	gaagcagtttggtgatttgt	tgaaaaatccacttatgagaca	201	55,60
14a	tgttctgaattgctgtcttg	ccttggagggggatgtag	311	56,61
14b	acagtcccaggctctaca	atgattctgcctaaatccaa	231	62,57
15	gcgtgtgtctcacctacttcc	ggtcatatacatatcaaacagtaattg	257	60
16	tgagaaagggtgttcagatt	tcttcagctacctccagaac	261	5358
17	tggaactaatttcaaatgccc	ccaccaaatacttacagggattc	169	54,59
18	ggatgatactccatctcccg	tcccagaaaagttaaagtcaaatc	350	57
19	tgtccttaaggcctctgtgc	cactccctggacatgtggac	190	60
20	acagttcaccccagtatggc	tgtgcataatcactggacaaca	174	59
21	tcctttggttagaacagcag	tcaagagaatgaaagggaaa	196	56
22	attgcaagttttcatttggt	ttccagagaaagtaacaacg	171	58
23	cggtttatctaagttgtgtaagca	ggcttcagataagttttgcca	160	54
24	aacaacagtaaatttgcacctca	tttggatccacgaggagag	241	54,59
25	gtggttcccccaccatct	cggagctagccactgtgag	205	60
26	tttcctcttcatttctcttca	ccagaatgccatgctagttaaa	239	55
27	ttccttaatgttctttctctttg	ttccagatctattgtcagca	242	54,59
28	catgcatgttttcacaggat	agaagtttcaaaggaaactcaatg	213	57
29	tttcttgtctcctttgtgctt	agaaatcttgccgtttttc	236	59
30	gaggccctgtctcaaaaa	ctcctccttcccacaacc	350	62
31a	gagagtttacgtgcacctcct	ccattggttttccgtttg	303	61
31b	atcctgccagaagatgaag	gttggtgtcgttggagtg	309	62
31c	gtggttgggcagcaacag	ctgctctctgaatctgcatt	289	63
31d	ctcagccaccccttccag	ttcaaaggttgaccatgc	337	57,62
31	gccagccatgatgtcagt	gcatggtaggtggctgta	320	62
31f	gctgttggctgcattcat	gatgtctcggaattgtgaag	310	62
31g	ctcagcagatgaacatgaac	catctgttgctgaaggagtc	314	61
31h	aggcaggtgccagtctac	agctaccagtccaggatg	305	62
31i	ctcagccttctccacacc	gctcccaaaatactacaagg	273	61

¹All primer sequences are shown in the 5' to 3' direction. ²The optimal melting temperature for each PCR fragment were calculated using the DHPLC Melt program http://insertion.stanford.edu/melt.html. Where more than one optimal temperature was predicted, both were used in the DHPLC analysis.

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ISSN: 1465-542X

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