Exon
|
Forward1
|
Reverse
|
Product size (base pairs)
|
Predicted melt temperature (°C)2
|
---|
1
|
Atttcctgaggattctggtt
|
cgcaccgagtagaaaagat
|
202
|
57,62
|
2a
|
gacctttgtcttttcccttt
|
accctgatttggtccacta
|
372
|
60
|
2b
|
aggcaggcttgacttctc
|
gcacacaaaccaaaactgta
|
372
|
60
|
3
|
ttgcttattttgttttcttttg
|
atgccagtttgaaaacaagt
|
254
|
52,57
|
4
|
agcacattatgactcctaccatt
|
acagtaaccctgtgggtcc
|
333
|
57,62
|
5
|
cattaacctgctcttgaaaaa
|
actggtgagactttaaaactt
|
210
|
57
|
6
|
ttctcaccagcattaatttgt
|
tgacacaaccaataccatgt
|
335
|
5560
|
7
|
tgtctcctgttatttcattttg
|
tgtaatgtgacccagggtat
|
161
|
57
|
8
|
aactatttggtgaccccttt
|
gaatgagacgtgtccacaat
|
193
|
54,59
|
9
|
aaatgctgacatgatattacagtgg
|
ccacaacaggttcaatcttgg
|
212
|
58
|
10
|
tgttacctggtggtagttcc
|
caaacagaaatataacaaaaacca
|
266
|
58,53
|
11
|
aatattttgtggggtttgtg
|
gtacaccggggattctttta
|
215
|
58,53
|
12
|
aatttcacaaaggcattcag
|
aggtaaaggccaaagagatt
|
196
|
55,60
|
13
|
gaagcagtttggtgatttgt
|
tgaaaaatccacttatgagaca
|
201
|
55,60
|
14a
|
tgttctgaattgctgtcttg
|
ccttggagggggatgtag
|
311
|
56,61
|
14b
|
acagtcccaggctctaca
|
atgattctgcctaaatccaa
|
231
|
62,57
|
15
|
gcgtgtgtctcacctacttcc
|
ggtcatatacatatcaaacagtaattg
|
257
|
60
|
16
|
tgagaaagggtgttcagatt
|
tcttcagctacctccagaac
|
261
|
5358
|
17
|
tggaactaatttcaaatgccc
|
ccaccaaatacttacagggattc
|
169
|
54,59
|
18
|
ggatgatactccatctcccg
|
tcccagaaaagttaaagtcaaatc
|
350
|
57
|
19
|
tgtccttaaggcctctgtgc
|
cactccctggacatgtggac
|
190
|
60
|
20
|
acagttcaccccagtatggc
|
tgtgcataatcactggacaaca
|
174
|
59
|
21
|
tcctttggttagaacagcag
|
tcaagagaatgaaagggaaa
|
196
|
56
|
22
|
attgcaagttttcatttggt
|
ttccagagaaagtaacaacg
|
171
|
58
|
23
|
cggtttatctaagttgtgtaagca
|
ggcttcagataagttttgcca
|
160
|
54
|
24
|
aacaacagtaaatttgcacctca
|
tttggatccacgaggagag
|
241
|
54,59
|
25
|
gtggttcccccaccatct
|
cggagctagccactgtgag
|
205
|
60
|
26
|
tttcctcttcatttctcttca
|
ccagaatgccatgctagttaaa
|
239
|
55
|
27
|
ttccttaatgttctttctctttg
|
ttccagatctattgtcagca
|
242
|
54,59
|
28
|
catgcatgttttcacaggat
|
agaagtttcaaaggaaactcaatg
|
213
|
57
|
29
|
tttcttgtctcctttgtgctt
|
agaaatcttgccgtttttc
|
236
|
59
|
30
|
gaggccctgtctcaaaaa
|
ctcctccttcccacaacc
|
350
|
62
|
31a
|
gagagtttacgtgcacctcct
|
ccattggttttccgtttg
|
303
|
61
|
31b
|
atcctgccagaagatgaag
|
gttggtgtcgttggagtg
|
309
|
62
|
31c
|
gtggttgggcagcaacag
|
ctgctctctgaatctgcatt
|
289
|
63
|
31d
|
ctcagccaccccttccag
|
ttcaaaggttgaccatgc
|
337
|
57,62
|
31
|
gccagccatgatgtcagt
|
gcatggtaggtggctgta
|
320
|
62
|
31f
|
gctgttggctgcattcat
|
gatgtctcggaattgtgaag
|
310
|
62
|
31g
|
ctcagcagatgaacatgaac
|
catctgttgctgaaggagtc
|
314
|
61
|
31h
|
aggcaggtgccagtctac
|
agctaccagtccaggatg
|
305
|
62
|
31i
|
ctcagccttctccacacc
|
gctcccaaaatactacaagg
|
273
|
61
|
-
1All primer sequences are shown in the 5' to 3' direction. 2The optimal melting temperature for each PCR fragment were calculated using the DHPLC Melt program http://insertion.stanford.edu/melt.html. Where more than one optimal temperature was predicted, both were used in the DHPLC analysis.