Figure 2

Sodium bisulfite verification of amplification of intermethylated sites (AIMS) protocol. (a) AIMS gel of untreated (-) MCF-7 cells and cells cultured in the presence (+) of 1 μmol/l 5-azacytidinecytidine for 96 hours. Arrow indicates the band that was excised, cloned and sequenced following sodium bisulfite analysis. The methylation status of the SmaI/PspAI restriction enzyme site was confirmed by sequencing the sodium bisulfite converted DNA from the (b) control and (c) 1 μmol/l 5-azacytidine-treated MCF-7 DNA cells.