Figure 1From: Chemically induced DNA hypomethylation in breast carcinoma cells detected by the amplification of intermethylated sitesFluorogram of amplification of intermethylated sites (AIMS) gel showing human breast cancer cell lines treated with 1 μmol/l 5-azacytidine for 48, 72 and 96 hours (+): (a) MCF-7, .(b) HCC1806, (c) MDA-MB231 and (d) MDA-MB468. Profiles of bands from different experiments are highly reproducible with any particular cell line, whereas banding patterns of individual cell lines vary considerably. Banding patterns differ as the culture period is extended, reflecting increased hypomethylation of genomic DNA. Arrows indicate decreases in or loss of band intensity, which are indicative of hypomethylation. The numbered bands are those that were excized, further characterized and localized to specific sites in the genome (see Table 1). Arrowheads indicate loss of bands and hypomethylation that is apparently dependent on culture conditions.Back to article page