Figure 4
From: The Icelandic founder mutation BRCA2 999del5: analysis of expression
Detection of BRCA2 999del5 protein under denaturing conditions and after proteasome inhibition. (a) COS7 cells were transfected with pIND-BRCA2 999del5 or pIND-LacZ without proteasome inhibition. Samples were treated under denaturing conditions. (b) COS7 cells were transfected with pIND-BRCA2 999del5 or pIND-LacZ with proteasome inhibition. Samples were purified with nickel-coated agarose beads under denaturing conditions. Samples in both (a) and (b) were blotted with an antibody against the V5 tag (α-V5).