Transforming growth factor (TGF)-β1 did not induce epithelial to mesenchymal transition in Colo357, Panc-1, or HaCaT Cells. Phase contrast images of (a,e) Colo357, (i,m) Panc-1, and (q,u) HaCaT cells treated with (TGF-β1) or without (control) 5 ng/ml TGF-β1 for 48 hours. Immunofluorescence detection of (b,f,j,n,r,v) E-cadherin, (c,g,k,o,s,w) zonula occludens (ZO)-1, and (d,h,l,p,t,x) F-actin in Colo357, Panc-1, and HaCaT cells treated with (TGF-β1) or without (control) 5 ng/ml TGF-β1 for 48 hours. TGF-β1 treatment induced cell enlargement and formation of actin stress fibers in the Colo357 cells. However, junctional E-cadherin and ZO-1 remained intact after TGF-β1 treatment. Changes in cell morphology, E-cadherin, ZO-1, or actin localization were not observed in the Panc-1 and HaCaT cells. Micrographs are representative of two experiments.