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Table 2 List of the primers used for pyrosequencing

From: A screen for germline mutations in the gene encoding CCCTC-binding factor (CTCF) in familial non-BRCA1/BRCA2 breast cancer

Exons

Sense (5'–3') positions of primersa

Antisense (5'–3') positions of primersb

Sequencing positions of primers

5' UTR

188AAGAACAAGATGCGCTAG205

Biotin-259CTCCGTGGCTGCAAAGTCAGTT280

216GCTGACCAGGGGCTTGAGAGCTGGG239

4

723TCACATTCGCTCTCATACTGG742

Biotin-968CGGAGAAGCATTATCAATTC949

758AGTGCAGTTTGTGCAGTTATGC779

  1. aPosition according to GenBank reference cDNA sequence NM_006565. bPosition according to GenBank reference DNA sequence AF145470. UTR, untranslated region.