A screen for germline mutations in the gene encoding CCCTC-binding factor (CTCF) in familial non-BRCA1/BRCA2 breast cancer

Table 1 List of the primers used for amplification and cycle sequencing in the polymerase chain reaction

Fragments	Sense (5'–3') positions of primers^a	Antisense (5'–3') positions of primers	Size (bp)
1	¹⁶²GGAGAATGATTACGGACCTG¹⁸¹	⁶²²CTATGTTTATGGGCTGTTCCTC⁶⁰¹	461
2	⁵¹⁹AGTAATGGAGGGCACAGTG⁵³⁷	¹⁰¹⁷CGCATTAACCTCTGATAGCA⁹⁹⁸	499
3	⁹⁴⁰GAGGGCAAAGATGTAGATGTG⁹⁶⁰	¹⁴¹⁵CCAGTATGAGAGCGAATGTGA¹³⁹⁵	476
4	¹³⁶⁶GCCAGTGTAGAAGTCAGCAA¹³⁸⁵	¹⁸¹¹TCCTGTCTACAAGCGTAATCA¹⁷⁹¹	446
5	¹⁷⁶⁸AAGCGCTTTAAGTGTGACCAG¹⁷⁸⁸	²¹⁸⁴TTCTACGGCAGGCTCCTC²¹⁶⁷	417
6	²⁰⁴⁴GGGGAAAAGGAGGAGAA²⁰⁶¹	²⁵²⁰TAAACACAGCCCAGAGAAGTC²⁵⁰⁰	477

^aPosition according to GenBank reference cDNA sequence NM_006565. bp, base pairs.

ISSN: 1465-542X