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Figure 5 | Breast Cancer Research

Figure 5

From: Transforming growth factor-β induces Cdk2 relocalization to the cytoplasm coincident with dephosphorylation of retinoblastoma tumor suppressor protein

Figure 5

Transforming growth factor-β (TGF-β) and rapamycin cooperate to inhibit nuclear Cdk2 activity specifically. NMuMG cytoplasmic (C) or nuclear (N) extracts from cells treated as in Fig. 1 were subjected to immunoprecipitation (IP) with anti-Cdk2 antibody or antibody from preimmune serum (Rab. IgG) as in Fig. 4. The immunoprecipitates were subjected to immune-complex kinase assays with histone H1 as the substrate. The kinase reactions/immunoprecipitates were analyzed by phosphorimaging (histone H1 kinase assay) and by immunoblotting (IB) with antibodies specific for Cdk2, p21, or p27. The bar graph in the lower panel is a quantification of the histone H1 kinase assays. Cont., control; T, 10 ng/ml TGF-β1; R, 100 nM rapamycin.

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