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Figure 2 | Breast Cancer Research

Figure 2

From: Transforming growth factor-β induces Cdk2 relocalization to the cytoplasm coincident with dephosphorylation of retinoblastoma tumor suppressor protein

Figure 2

TGF-β and rapamycin together induce the dephosphorylation of nuclear Rb. The cytoplasmic (C) and nuclear (N) extracts prepared in Fig. 1a were analyzed by immunoblotting with antibodies specific for Rb phosphorylated on Thr821 (P-Rb(Thr821)), Rb phosphorylated on Ser249/Thr252 (P-Rb(Ser249/Thr252)), and total Rb. Where two bands of total Rb are apparent, the upper band represents hyperphosphorylated Rb and the lower band hypophosphorylated Rb. Cont., control; T + R, 10 ng/ml TGF-β1 plus 100 nM rapamycin; HMEC, primary human mammary epithelial cells.

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