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Figure 9 | Breast Cancer Research

Figure 9

From: Malignant myoepithelial cells are associated with the differentiated papillary structure and metastatic ability of a syngeneic murine mammary adenocarcinoma model

Figure 9

Secretion of proteases in vitro by the LM38 sublines. (a) Quantification of secreted urokinase-type plasminogen activator (uPA) activity by radial caseinolysis. The LM38-HP cells and LM38-D2 clone secreted significantly less uPA than LM38-LP cells. (b) Upper panel: zymogram of media conditioned by LM38-LP, LM38-HP, and LM38-D2 cells showing a main 48 kDa uPA band, confirming the differential activity observed in the radial assay. Lower panel: gelatin co-polymerized zymogram showing that only the highly metastatic LM38-LP cells were able to secrete matrix metalloproteinase (MMP)-2 to the culture medium. *P < 0.05 compared with LM38-LP.

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Breast Cancer Research

ISSN: 1465-542X

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