Figure 6From: Malignant myoepithelial cells are associated with the differentiated papillary structure and metastatic ability of a syngeneic murine mammary adenocarcinoma modelHistopathology of subcutaneous tumors formed after the inoculation of LM38 sublines into syngeneic mice. Tumor sections were stained with hematoxylin and eosin (upper panels) or by an immunohistochemistry procedure, for α-smooth muscle actin (α-SMA; middle panels), for pancytokeratins (pan-CK; left and central lower panels) or for cytokeratin 14 (CK14; right lower panel). The LM38-LP cells (left panels) formed differentiated papillary adenocarcinomas made up of cells positive for α-SMA and pan-CK surrounding fibrovascular strands. In contrast, the LM38-HP cells (central panels) formed poorly differentiated adenocarcinomas with no evidence of glandular structures, that were made up of cells mostly negative for both cell lineage markers. The LM38-D2 clone (right panels) grew as an undifferentiated tumor consisting of large cells positive for CK14 and α-SMA. (Original magnification ×400; scale bar, 35 μm.) E, epidermis; F, hair follicle; N, necrosis; S, fibrovascular stroma; T, tumor tissue.Back to article page