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Figure 2 | Breast Cancer Research

Figure 2

From: WISP3 and RhoC guanosine triphosphatase cooperate in the development of inflammatory breast cancer

Figure 2

Inhibition of WISP3 induces anchorage-independent growth, proliferation and secretion of vascular endothelial growth factor (VEGF) in human mammary epithelial (HME) cells. (a) Inhibition of WISP3 expression in HME cells. HME cells greatly increased the number of colonies formed in soft agar in comparison with empty vector control (HME/Flag; t-test, P < 0.05 for both clones). (b) Effect of inhibition of WISP3 expression on the proliferation of HME cells was studied with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The stable HME/AS WISP3 cells have a significant increase in the proliferation rate in comparison with the empty vector control. Results are expressed as means ± SEM of three independent experiments. In all, 3000 cells were assessed in each plate (t-test, P < 0.05). (c) Increase in VEGF measured by enzyme-linked immunosorbent assay, as a result of inhibition of WISP3 expression in HME cells. Results are expressed as means ± SEM; t-test, P < 0.05 for all clones.

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